ION BLUE Encyclopedia

GHK

GHK is the tripeptide Gly-His-Lys (copper-free form) studied in skin and wound-healing research; most functional data concern the copper complex GHK-Cu.

Evidence: Preclinical

Reading time:2 min
Citations:2
Updated:July 5, 2026

Type

Peptide

INCI Name

Tripeptide Glycyl-L-histidyl-L-lysine (Gly-His-Lys), the copper-free form of the GHK tripeptide

Direct Answer

GHK is the tripeptide Gly-His-Lys (copper-free form) studied in skin and wound-healing research; most functional data concern the copper complex GHK-Cu.

Summary Table

Evidence Level

Preclinical

AI Summary

GHK is a naturally occurring tripeptide (Gly-His-Lys) found in human plasma; this entry covers the copper-free form (the copper complex, GHK-Cu, is covered separately). The evidence base is preclinical: review and laboratory literature describe proposed roles in skin remodeling, wound healing, and gene expression, but much of the functional data concerns the copper-bound complex rather than the copper-free peptide, and independent controlled human outcome trials of the copper-free form are limited. Framed as studied, not established for defined clinical outcomes.

Key Information

Classification

2 Mechanisms

Key Takeaways

  • GHK is the endogenous tripeptide Gly-His-Lys (copper-free form); the copper complex GHK-Cu is covered separately
  • Most functional evidence concerns GHK-Cu rather than the copper-free peptide
  • It is used as a cosmetic ingredient, not an approved medicine, and human outcome data are limited

Scientific Overview

In Plain English

GHK is a small natural peptide (three amino acids) found in the blood that declines with age. Research, mostly in the lab, has looked at its role in skin and wound repair. Most of the functional studies actually use the copper-bound version (GHK-Cu). It is used as a cosmetic ingredient rather than an approved medicine.

Scientific Details

GHK (Gly-His-Lys) is an endogenous tripeptide present in human plasma that decreases with age. Review and laboratory literature describe proposed roles in tissue remodeling, wound healing, antioxidant activity, and modulation of gene expression. Much of this literature concerns the copper complex GHK-Cu, which binds copper with high affinity; evidence specific to the copper-free peptide, and independent controlled human outcome data, are limited.

How It Works

GHK is described in research as a copper-binding tripeptide associated with tissue-remodeling and gene-expression effects in laboratory models. The copper complex (GHK-Cu) is the form most studied for these activities. These mechanisms are characterized preclinically and are not confirmed by controlled human outcome studies for the copper-free peptide.

Mechanism of Action

Copper binding

cell

GHK binds copper(II) to form GHK-Cu; much of the peptide's described biological activity is attributed to the copper complex.

Gene-expression modulation

cell

Review and laboratory literature associate GHK/GHK-Cu with changes in expression of genes related to tissue remodeling and repair.

Evidence Level

Human Evidence

Human evidence largely concerns the copper complex (GHK-Cu) in topical/cosmetic contexts; independent controlled human outcome data specific to the copper-free peptide are limited.

Animal Evidence

Animal wound-healing studies describe tissue-remodeling effects, generally with the copper complex.

Cell Evidence

In vitro studies describe gene-expression and remodeling effects.

Limitations

Most functional evidence concerns GHK-Cu rather than the copper-free peptide; controlled human outcome trials are limited, and effects are not established for defined clinical endpoints.

Why This Grade

Graded preclinical: evidence is largely review- and laboratory-based, concerns the copper complex more than the copper-free peptide, and lacks independent controlled human outcome data.

References

  1. The potential of GHK as an anti-aging peptide. Aging Pathobiology and Therapeutics.Reviewdoi:10.31491/apt.2020.03.014
  2. Topically applied GHK as an anti-wrinkle peptide: Advantages, problems and prospective. BioImpacts.Reviewdoi:10.34172/bi.30071

Alternative Names

  • Glycyl-L-histidyl-L-lysine
  • Gly-His-Lys
  • Tripeptide-1
  • GHK (copper-free)

Risks & Safety

  • Not an approved medicine; used as a cosmetic ingredient with the usual patch-test considerations
  • Evidence specific to the copper-free peptide is limited; most data concern GHK-Cu
  • Injectable or systemic use is not supported by controlled human safety data

Claim Boundaries

ION BLUE is an educational research aggregator. This content summarizes published scientific literature. It is not medically reviewed, is not medical advice, and is not a recommendation to use any substance. Consult a licensed healthcare provider. This entry summarizes research on the GHK tripeptide and is not a recommendation to use it for any clinical purpose. See copper-tripeptide-1 for the copper complex.

This page summarizes published research and is for informational purposes only; it is not medical advice.